5O38

Human Brd2(BD2) mutant in free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9174
Spacegroup name	P 21 21 2
Unit cell lengths	52.420, 71.409, 31.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.499 - 1.200
R-factor	0.1189
Rwork	0.118
R-free	0.14130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qeu
RMSD bond length	0.005
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	1.243
High resolution limit [Å]	1.200	1.200
Rmerge	0.022	0.084
Number of reflections	37657	3520
<I/σ(I)>	15.57	6.62
Completeness [%]	98.3	93.37
Redundancy	2	1.9
CC(1/2)	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M Tris pH8.0 50% Pentaerythritol propoxylate (5/4 PO/OH) 0.2M Imidazole