5O32
The structure of complement complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 1 |
| Unit cell lengths | 116.370, 122.790, 157.200 |
| Unit cell angles | 69.76, 76.10, 70.13 |
Refinement procedure
| Resolution | 49.134 - 4.206 |
| R-factor | 0.230193326461 |
| Rwork | 0.229 |
| R-free | 0.25407 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS (20161101) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.200 |
| High resolution limit [Å] | 4.200 |
| Rmerge | 0.174 |
| Rpim | 0.109 |
| Number of reflections | 55430 |
| <I/σ(I)> | 6.1 |
| Completeness [%] | 93.4 |
| Redundancy | 3.5 |
| CC(1/2) | 0.989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 60mM sodium malonate PH5.0 6% w/v PEG3350 |
