5NWZ

FIBROBLAST GROWTH FACTOR RECEPTOR 4 KINASE DOMAIN (449-753) IN COMPLEX WITH IRREVERSIBLE LIGAND CGA159527

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	47.398, 75.958, 181.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.960 - 2.370
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.960	2.430
High resolution limit [Å]	2.370	2.370
Rmerge	0.110	1.209
Number of reflections	27374
<I/σ(I)>	13.66	1.65
Completeness [%]	99.9	99.9
Redundancy	6.53	6.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	TRIS, 25% PEG3350. 1MM EDTA, PH8.0