5NT7
Structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2014-11-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97908 |
Spacegroup name | P 1 |
Unit cell lengths | 38.950, 39.040, 97.480 |
Unit cell angles | 89.28, 91.85, 99.26 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.048 | 1.382 |
Number of reflections | 102457 | |
<I/σ(I)> | 10.44 | 0.74 |
Completeness [%] | 91.4 | 87.5 |
Redundancy | 2.16 | 2.18 |
CC(1/2) | 0.394 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Tris pH 7.5, 200 mM potassium thiocyanate, 16% PEG 3350 |