5NPZ

Porcine (Sus scrofa) Major Histocompatibility Complex, class I, presenting EFEDLTFLA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92819
Spacegroup name	P 1 21 1
Unit cell lengths	42.034, 128.290, 46.794
Unit cell angles	90.00, 101.90, 90.00

Refinement procedure

Resolution	41.170 - 1.430
R-factor	0.1855
Rwork	0.184
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PHASER
RMSD bond length	0.019
RMSD bond angle	1.801
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.750	42.750	1.470
High resolution limit [Å]	1.430	6.400	1.430
Rmerge	0.058	0.021	0.881
Rmeas	0.068	0.025	1.049
Rpim	0.035	0.012	0.562
Number of reflections	89178
<I/σ(I)>	10.7
Completeness [%]	99.9	99.7	99.7
Redundancy	3.6	4	3.1
CC(1/2)	0.998	0.999	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	TOPS screen, condition B02: 20% PEG 4000, 15% Glycerol, 0.1 M Sodium Cacodylate, pH 6.5