5NPV

Structure of human ATG5-ATG16L1(ATG5BD) complex (I4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-26
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	I 4
Unit cell lengths	143.580, 143.580, 62.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.404 - 3.100
R-factor	0.2543
Rwork	0.253
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gdk
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.404	45.404	3.200
High resolution limit [Å]	3.100	10.000	3.100
Rmerge	0.167	0.025	0.723
Rmeas	0.185	0.028	0.799
Number of reflections	11680	360	1069
<I/σ(I)>	11.45	49.09	2.22
Completeness [%]	99.6	94.5	100
Redundancy	5.51	4.956	5.547
CC(1/2)	0.992	0.999	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.76	293	0.1 M MES pH 5.76, 0.378 M KCl, 21.7 % PEG 3350