5NOI

human Robo2 extracellular domains 4-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.008818
Spacegroup name	I 41 2 2
Unit cell lengths	99.749, 99.749, 123.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.800 - 2.400
R-factor	0.2401
Rwork	0.237
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c4k 2edj
RMSD bond length	0.023
RMSD bond angle	1.915
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.800
High resolution limit [Å]	2.400
Number of reflections	12530
<I/σ(I)>	21.6
Completeness [%]	100.0
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.1M NaCl, 11-13% PEG 6000