5NN8
Crystal structure of human lysosomal acid-alpha-glucosidase, GAA, in complex with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.872600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.050, 102.986, 127.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.550 - 2.450 |
| R-factor | 0.17611 |
| Rwork | 0.174 |
| R-free | 0.21231 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5nn3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.550 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.188 | 0.885 |
| Rpim | 0.116 | 0.554 |
| Number of reflections | 44773 | 4760 |
| <I/σ(I)> | 8 | 1.9 |
| Completeness [%] | 94.7 | 97 |
| Redundancy | 5.3 | 5.2 |
| CC(1/2) | 0.986 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.9 M ammonium sulphate, 0.1 M HEPES pH 7.0, 2% v/v PEG400 |
