5NMR

Monomeric mouse Sortilin extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99998
Spacegroup name	C 1 2 1
Unit cell lengths	200.580, 62.570, 67.310
Unit cell angles	90.00, 101.35, 90.00

Refinement procedure

Resolution	65.994 - 2.100
R-factor	0.2015
Rwork	0.200
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f6k
RMSD bond length	0.003
RMSD bond angle	1.470
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	66.000
High resolution limit [Å]	2.000
Rpim	0.090
Number of reflections	46680
<I/σ(I)>	6.3
Completeness [%]	97.5
Redundancy	3.1
CC(1/2)	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Na HEPES pH 7.5, 1 mM CaCl2 and 25% PEG 2000 MME (w/v).