5NLE
Chicken GRIFIN (crystallisation pH: 8.0)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.632, 103.976, 130.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.210 - 1.845 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nld |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.210 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.021 | 0.470 |
| Number of reflections | 54344 | 5238 |
| <I/σ(I)> | 14.12 | 1.66 |
| Completeness [%] | 99.8 | 97.92 |
| Redundancy | 2 | |
| CC(1/2) | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 10% PEG 3K, 0.1M Imidazole pH 8.0, 0.2M Li2SO4 |
