5NL0

Crystal structure of a 197-bp palindromic 601L nucleosome in complex with linker histone H1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	C 2 2 21
Unit cell lengths	61.725, 405.737, 348.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.128 - 5.400
R-factor	0.2422
Rwork	0.240
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut9
RMSD bond length	0.008
RMSD bond angle	1.334
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	6.040
High resolution limit [Å]	5.400	5.400
Rmerge	0.091	0.685
Rpim	0.072	0.553
Number of reflections	15641	4370
<I/σ(I)>	9.1	2.1
Completeness [%]	99.5	99.6
Redundancy	4.5	4.4
CC(1/2)	0.997	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293.15	Mix of equal volumes of the nucleosome/H1 complex (25-30 microM) and a crystallization solution composed of MPD (6% v/v), 50 mM NaCl, and 50 mM sodium potassium phosphate pH 6.4.