5NKM
SMG8-SMG9 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.085, 111.085, 374.474 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.412 - 2.493 |
| R-factor | 0.2264 |
| Rwork | 0.225 |
| R-free | 0.26020 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.360 | 2.583 |
| High resolution limit [Å] | 2.493 | 2.493 |
| Rmerge | 0.137 | 2.587 |
| Number of reflections | 188934 | |
| <I/σ(I)> | 27.25 | |
| Completeness [%] | 98.0 | |
| Redundancy | 39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 285 | 10% PEG3350, 0.1M Tris pH 8.5 |
