5NJD

Structure of Interleukin 23 in complex with Briakinumab FAb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	191.606, 191.606, 519.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.800 - 3.900
R-factor	0.2724
Rwork	0.272
R-free	0.31180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	composite model 4OE8 and homology model for FAb
RMSD bond length	0.004
RMSD bond angle	0.552
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.800	94.810	4.140
High resolution limit [Å]	3.900	11.640	3.900
Rmeas	0.438	0.085	1.378
Number of reflections	86556	3549	13014
<I/σ(I)>	4.2	16.02	1.18
Completeness [%]	97.7	95.9	92.5
Redundancy	5.1	4.61	5.12
CC(1/2)	0.960	0.995	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	287	2 M (NH4)2SO4, 100 mM BisTris