5NG2

Structure of RIP2K(D146N) with bound Staurosporine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.972
Spacegroup name	P 41 21 2
Unit cell lengths	96.083, 96.083, 202.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.850 - 2.800
R-factor	0.20931
Rwork	0.208
R-free	0.22562
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ng0
RMSD bond length	0.008
RMSD bond angle	1.732
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.040
High resolution limit [Å]	2.800
Rmerge	0.192
Number of reflections	22957
<I/σ(I)>	8.8
Completeness [%]	99.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	.1 M MES pH6.5, 2M NaCl, 0.1 mM Na(H2PO4), 0.1 mM of K(H2PO4)