5NFP
Glucocorticoid Receptor in complex with budesonide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-04-26 |
| Detector | ADSC HF-4M |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.990, 83.990, 106.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.860 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.900 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 25028 | 1812 |
| <I/σ(I)> | 15.6 | 2.9 |
| Completeness [%] | 97.5 | 96.6 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2 ul of protein, 0.2ul of Polypropylene Glycol 400 and 0.8ul of well solution (10-12% PEG8000, 20% ethylene glycol and 0.1 M Hepes pH 7.5). |
