5NFJ

Crystal structure of the methyltransferase subunit of human mitochondrial Ribonuclease P (MRPP1) bound to S-adenosyl-methionine (SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92820
Spacegroup name	P 43
Unit cell lengths	82.640, 82.640, 148.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.640 - 1.960
R-factor	0.18649
Rwork	0.185
R-free	0.20648
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.878
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	82.640
High resolution limit [Å]	1.960
Rmerge	0.052
Number of reflections	67692
<I/σ(I)>	16.95
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	Well solution: 12% PEG 1000, 28% glycerol, 1.5%(w/v) PEG 3350 Cryo: 25% propylene glycol Protein concentration: 11 mg/mL