5NA9
The X-ray structure of bovine pancreatic ribonuclease incubated in the presence of an excess of carboplatin (1:10 ratio)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-22 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.168, 64.168, 64.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.570 - 2.070 |
| R-factor | 0.182 |
| Rwork | 0.178 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rnx |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.570 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.109 | 0.355 |
| Number of reflections | 9665 | |
| <I/σ(I)> | 13.6 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.5 M ammonium sulfate - 0.1 M sodium acetate pH 5.5 - 3.0 M cesium chloride |
