5N86
Crystal structure of FAS1 domain of hyaluronic acid receptor stabilin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.740, 55.830, 87.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.069 - 1.484 |
| R-factor | 0.1464 |
| Rwork | 0.145 |
| R-free | 0.17170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o70 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.013 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.069 | 48.740 | 1.560 |
| High resolution limit [Å] | 1.484 | 4.690 | 1.484 |
| Rmerge | 0.068 | 0.183 | |
| Rmeas | 0.094 | 0.075 | 0.201 |
| Rpim | 0.038 | 0.031 | 0.082 |
| Number of reflections | 40147 | ||
| <I/σ(I)> | 10.9 | 7.9 | 3.2 |
| Completeness [%] | 99.8 | 99.7 | 100 |
| Redundancy | 5.7 | 5.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 0.8 M sodium/potassium tartrate, 0.5% PEG 5000 MME |
