5N2W

WT-Parkin and pUB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 63 2 2
Unit cell lengths	147.290, 147.290, 87.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.340 - 2.680
R-factor	0.201
Rwork	0.198
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c1z 5caw
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.340	2.810
High resolution limit [Å]	2.680	2.680
Rmerge	0.107	0.719
Number of reflections	15028
<I/σ(I)>	8.9	1.9
Completeness [%]	93.6	93.7
Redundancy	4.6	4.2
CC(1/2)		0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100mM Tris pH 8.5, 200mM TMAO, PEG MME 2000