5N27

X-ray structure of human heavy chain ferritin (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	182.955, 182.955, 182.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.630 - 1.740
R-factor	0.14823
Rwork	0.146
R-free	0.19146
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y08
RMSD bond length	0.020
RMSD bond angle	1.749
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.630	1.770
High resolution limit [Å]	1.740	1.740
Rmerge	0.114	0.733
Rpim	0.032	0.404
Number of reflections	27459
<I/σ(I)>	18	1.8
Completeness [%]	99.9	98.4
Redundancy	12.2	3.8
CC(1/2)		0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.0 M MgCl2, 0.1 M bicine pH 9.0