5MZV

IL-23:IL-23R:Nb22E11 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	67.219, 112.190, 109.870
Unit cell angles	90.00, 106.24, 90.00

Refinement procedure

Resolution	76.851 - 2.800
R-factor	0.2137
Rwork	0.212
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	hybrid model of IL23 from 4OE8 in complex with nanobody 22E11 from 4GRW
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.000	2.870
High resolution limit [Å]	2.800	2.800
Rmeas	0.081	0.547
Number of reflections	38061	2797
<I/σ(I)>	12.45	2.71
Completeness [%]	98.4	99.1
Redundancy	3.45	3.53
CC(1/2)	0.997	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	287	200 mM magnesium chloride, 100 mM Tris, 11 % (w/v) PEG8000