5MYY
Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.740, 78.740, 37.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.680 - 1.100 |
| R-factor | 0.13013 |
| Rwork | 0.130 |
| R-free | 0.14305 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.370 |
| High resolution limit [Å] | 1.100 |
| Number of reflections | 47744 |
| <I/σ(I)> | 55 |
| Completeness [%] | 100.0 |
| Redundancy | 28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Sodium acetate pH 4.8 10-12% NaCl 20-25% Ethylene Glycol 60 mg/ ml HEWL in 0.1 M Sodium acetate pH 4.8 |
