5MYO
Structure of Pyroglutamate-Abeta-specific Fab c#6 in complex with human Abeta-pE3-12-PEGb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.774, 65.958, 67.760 |
| Unit cell angles | 62.87, 82.93, 84.11 |
Refinement procedure
| Resolution | 27.189 - 1.590 |
| R-factor | 0.1796 |
| Rwork | 0.178 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zea |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.189 | 30.000 | 1.690 |
| High resolution limit [Å] | 1.590 | 27.189 | 1.590 |
| Rmerge | 0.034 | 0.333 | |
| Rmeas | 0.041 | 0.458 | |
| Number of reflections | 99426 | 10110 | |
| <I/σ(I)> | 17.77 | 2.03 | |
| Completeness [%] | 89.3 | 54.3 | |
| Redundancy | 2.807 | 1.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 286 | 25.5% w/v PEG 4000 15% glycerol 170 mM ammonium sulfate |
