5MYA
Homodimerization of Tie2 Fibronectin-like domains 1-3 in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.160, 104.690, 79.520 |
| Unit cell angles | 90.00, 121.41, 90.00 |
Refinement procedure
| Resolution | 29.419 - 2.900 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.27720 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 20882 | 3444 |
| <I/σ(I)> | 16.1 | 1.5 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 11.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris buffer at pH 7.0 - 8.5 and 14-20% PEG 3350 (w/v) |
