5MX0
Crystal structure of human fibromodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.110, 98.930, 111.340 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 65.913 - 2.210 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xku |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.759 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.913 | 2.270 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.111 | 1.840 |
| Rpim | 0.046 | 0.737 |
| Number of reflections | 55434 | |
| <I/σ(I)> | 9.5 | 1.1 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.998 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 10 mg/ml protein + 26% (w/v) PEG 3350, 200 mM lithium sulphate monohydrate, 100 mM Tris-HCl (pH 8.5) |
