5MWH

Crystal structure of the human BRPF1 bromodomain in complex with BZ089

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000019
Spacegroup name	P 1 21 1
Unit cell lengths	48.689, 63.279, 49.000
Unit cell angles	90.00, 102.15, 90.00

Refinement procedure

Resolution	38.190 - 1.650
R-factor	0.1817
Rwork	0.179
R-free	0.22560
RMSD bond length	0.006
RMSD bond angle	0.719
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.190
High resolution limit [Å]	1.650
Rmerge	0.040
Number of reflections	32567
<I/σ(I)>	21.3
Completeness [%]	93.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium nitrate, 20% PEG3350