5MWB
Human Notch-2 EGF11-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 20.160, 49.790, 125.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.003 - 1.860 |
| R-factor | 0.2298 |
| Rwork | 0.228 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.550 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.800 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.077 | 1.067 |
| Number of reflections | 11247 | |
| <I/σ(I)> | 11.2 | 1.3 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 5.2 | 5.1 |
| CC(1/2) | 0.998 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 8000, Sodium acetate, Sodium cacodylate |
