5MW2
CRYSTAL STRUCTURE OF BCL-6 BTB-domain with BI-3802
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 31.367, 72.657, 54.883 |
| Unit cell angles | 90.00, 105.87, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.28300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.6)) |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.790 | 2.365 |
| High resolution limit [Å] | 2.350 | 2.355 |
| Number of reflections | 4788 | |
| <I/σ(I)> | 10.6 | 2.1 |
| Completeness [%] | 96.6 | 97.2 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 296 | 22.5 % PEG 3350 2 % isopropanol 0.1 M Calcium cloride 0.1 M HEPES |
