5MVZ
Fab 4AB007 bound to Interleukin-1-beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000, 2.066 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.041, 158.385, 153.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.680 - 2.150 |
| R-factor | 0.22253 |
| Rwork | 0.219 |
| R-free | 0.28107 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.677 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 2.210 |
| High resolution limit [Å] | 2.093 | 2.093 |
| Rmerge | 0.073 | |
| Rmeas | 0.085 | |
| Number of reflections | 79698 | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 95.2 | 94.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Protein mix: 10mg/ml Protein 10mM Imidazole buffer, pH=8.0 50mM NaCl Reservoir: 18% PEG4000 (w/v), 0.1M Hepes pH=7.0, 10mM ATP, Drop Ratio 1:1 |
