5MVR
Crystal structure of Bacillus subtilus YdiB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07252 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.590, 59.820, 39.340 |
| Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
| Resolution | 43.835 - 1.762 |
| R-factor | 0.1816 |
| Rwork | 0.180 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1htw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.840 |
| High resolution limit [Å] | 1.760 |
| Rmerge | 0.032 |
| Number of reflections | 14655 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 98.6 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 42 % PEG600 and 200 mM Imidazole malate pH 5.5 |
