5MUR
X-ray structure of the F14'A mutant of GLIC in complex with propofol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2011-11-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.281, 132.972, 158.976 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.1944 |
| Rwork | 0.194 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 66286 |
| <I/σ(I)> | 16.6 |
| Completeness [%] | 99.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Na acetate pH4 12-15% PEG4K 200 mM NaSCN 16% glycerol 2% DMSO |
