5MUO
X-ray structure of the 2-22' locally-closed mutant of GLIC in complex with propofol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-03-03 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.980, 132.900, 159.920 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.190 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tlv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.230 | 3.050 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.677 |
| Number of reflections | 78570 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K |
