5MQ4

Crystal Structure of the leucine zipper of human PRKCBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	C 2 2 21
Unit cell lengths	66.638, 237.049, 210.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.625 - 2.700
R-factor	0.2096
Rwork	0.208
R-free	0.23620
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.106
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.150	2.790
High resolution limit [Å]	2.700	2.700
Rmerge		1.143
Number of reflections	46326
<I/σ(I)>	13.6	2.4
Completeness [%]	100.0	100
Redundancy	10	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	274	1M ammonium sulfate -- 0.1M MES pH 6.3