5MPN

Crystal structure of CREBBP bromodomain complexed with FA26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00001
Spacegroup name	P 21 21 21
Unit cell lengths	44.584, 44.723, 60.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.017 - 1.230
R-factor	0.1587
Rwork	0.158
R-free	0.16740
RMSD bond length	0.014
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.720
High resolution limit [Å]	1.230
Rmerge	0.058
Number of reflections	34316
<I/σ(I)>	16.5
Completeness [%]	95.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.2 M MgCl2, 0.1 M Bis-Tris, pH6.5, 5% EG, 23% PEG3350