5MPK

Crystal structure of CREBBP bromodomain complexed with DK19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	45.077, 60.204, 87.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.811 - 1.900
R-factor	0.1883
Rwork	0.183
R-free	0.23910
RMSD bond length	0.011
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.080
High resolution limit [Å]	1.900
Rmerge	0.091
Number of reflections	19464
<I/σ(I)>	12
Completeness [%]	99.9
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M HEPES-Na, pH7.5, 0.2 M MgCl2, 25% P3350