5MP3

Crystal structure of DC8E8 Fab in the complex with a 30-mer tau peptide at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-02-23
Detector	MAR555 FLAT PANEL
Wavelength(s)	0.815
Spacegroup name	P 1 21 1
Unit cell lengths	61.850, 89.890, 83.600
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	44.940 - 2.750
R-factor	0.2014
Rwork	0.199
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4OZ4
RMSD bond length	0.013
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.940	44.940	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.108	0.023	0.787
Rmeas	0.129
Total number of observations	82638
Number of reflections	23880
<I/σ(I)>	10.98	41.33	1.52
Completeness [%]	99.8	97.9	98.9
Redundancy	3.461	3.609	3.073
CC(1/2)	0.993	0.999	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% PEG 8000, 0.1 M Na-cacodylate, 0.2 M Mg-acetate