5MP1

Crystal structure of DC8E8 Fab in the complex with a 14-mer tau peptide at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	60.370, 82.370, 89.800
Unit cell angles	88.12, 84.78, 89.93

Refinement procedure

Resolution	39.830 - 3.100
R-factor	0.2245
Rwork	0.222
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4OZ4
RMSD bond length	0.010
RMSD bond angle	1.432
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.830	39.830	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.081	0.026	0.313
Number of reflections	24488
<I/σ(I)>	9.64	26.93	2.67
Completeness [%]	78.4	49.3	60.2
Redundancy	1.722	1.908	1.571
CC(1/2)	0.987	0.998	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	20% PEG 4000; 10% 2-Propanol, 0.1 M Na-HEPES