5MOK
Crystal structure of human IgE-Fc epsilon 3-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.478, 100.357, 77.856 |
Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
Resolution | 77.217 - 2.000 |
R-factor | 0.2036 |
Rwork | 0.202 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ha0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.619 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.360 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 68470 | |
<I/σ(I)> | 14 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 17.3 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% (v/v) PEG1500 0.1M SPG (succinic acid, sodium dihydrogen phosphate and glycine in the ratio 2:7:7) at pH 6.0 |