5MLZ

Dolichyl phosphate mannose synthase in complex with GDP and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	90.870, 146.210, 95.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.016 - 2.000
R-factor	0.2418
Rwork	0.241
R-free	0.25710
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.950	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	43116
<I/σ(I)>	29.2	2.2
Completeness [%]	99.8	99.5
Redundancy	13.3
CC(1/2)		0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2 M potassium chloride, 0.1 M trisodium citrate (pH 5.5), 37% (v/v) pentaerythritol propoxylate (5/4 PO/OH), 50 mM Hepes (pH 7.5), 150 mM NaCl, 10% (v/v) glycerol, 0.05% LDAO, 5 mM GDP, 5 mM MgCl2