5ML9
Cocrystal structure of Fc gamma receptor IIIa interacting with Affimer F4, a specific binding protein which blocks IgG binding to the receptor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.481, 72.587, 96.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.740 - 2.350 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A SUBSECTION OF A COMPLEX OF AN ADHIRON BOUND TO A SOLUBLE PROTEIN (MANUSCRIPT IN PREPARATION) WAS USED AS A SEARCH MODEL. |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.474 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | 2.430 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.148 | 1.108 |
| Number of reflections | 16390 | |
| <I/σ(I)> | 7.1 | 1.5 |
| Completeness [%] | 95.4 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 2.0 M Ammonium Sulphate 0.1 M Sodium Cacodylate pH=6.50 0.2 M Sodium Chloride |
