5ML9

Cocrystal structure of Fc gamma receptor IIIa interacting with Affimer F4, a specific binding protein which blocks IgG binding to the receptor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	56.481, 72.587, 96.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 2.350
R-factor	0.222
Rwork	0.219
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	A SUBSECTION OF A COMPLEX OF AN ADHIRON BOUND TO A SOLUBLE PROTEIN (MANUSCRIPT IN PREPARATION) WAS USED AS A SEARCH MODEL.
RMSD bond length	0.002
RMSD bond angle	0.474
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.500	2.430
High resolution limit [Å]	2.340	2.340
Rmerge	0.148	1.108
Number of reflections	16390
<I/σ(I)>	7.1	1.5
Completeness [%]	95.4
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	2.0 M Ammonium Sulphate 0.1 M Sodium Cacodylate pH=6.50 0.2 M Sodium Chloride