5MJJ
Single-shot pink beam serial crystallography: Lysozyme
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-10 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.1-1.3 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.100, 79.100, 37.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.014 - 1.750 |
| R-factor | 0.163947591569 |
| Rwork | 0.161 |
| R-free | 0.18829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fek |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.473 |
| Data reduction software | Precognition/Epinorm (RenzResearch) |
| Phasing software | PHENIX (1.10.1-2155_9999) |
| Refinement software | PHENIX (1.10.1-2155_9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.830 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.076 | |
| Number of reflections | 9538 | |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 75.5 | 25.21 |
| Redundancy | 9.2 | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277 | 1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5 |
