5MIV
G307E variant of murine Apoptosis Inducing Factor in complex with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.430, 116.780, 166.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.500 - 3.100 |
| R-factor | 0.20121 |
| Rwork | 0.198 |
| R-free | 0.26269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gd4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.536 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 26576 | |
| <I/σ(I)> | 6.6 | 1.3 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.5 | 4.7 |
| CC(1/2) | 0.992 | 0.331 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 6000, 0.1 M HEPES, pH 7.0, 1 mM NADH |
