5MIO

KIF2C-DARPIN FUSION PROTEIN BOUND TO TUBULIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	51.810, 229.760, 293.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.910 - 3.190
R-factor	0.213
Rwork	0.211
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eyp 2heh
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000
High resolution limit [Å]	3.190
Rmeas	0.210	2.930
Number of reflections	29646
<I/σ(I)>	5.34
Completeness [%]	97.3
Redundancy	3.56
CC(1/2)	0.995	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	40 mM Pipes-K pH 6.8 4% PEG3350