5MGV
Kinetic and Structural Changes in HsmtPheRS, Induced by Pathogenic Mutations in Human FARS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 295 |
| Detector technology | PIXEL |
| Collection date | 2016-02-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.770, 90.370, 95.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.050 |
| Rwork | 0.228 |
| R-free | 0.27110 |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.050 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 28291 |
| <I/σ(I)> | 25.22 |
| Completeness [%] | 100.0 |
| Redundancy | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 2 mM DTT, 100 mM bis-Tris propane pH 7 and 1.8 M sodium acetate pH 7 |
