5MFE
Designed armadillo repeat protein YIIIM5AII in complex with (RR)4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.000, 103.673, 134.182 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 1.950 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mfc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.241 | 3.680 |
| Number of reflections | 83955 | |
| <I/σ(I)> | 7.94 | 0.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.6 | 8.8 |
| CC(1/2) | 0.155 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | PEG8000 5%, MPD 27.27%, NaCacodylate 0.1M pH7 200mM CaCl2 |
