5ME3
Structure of the Scc2 C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.049, 106.440, 143.183 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 48.810 - 2.850 |
| R-factor | 0.2201 |
| Rwork | 0.217 |
| R-free | 0.26910 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.862 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 2.952 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.071 | 1.244 |
| Number of reflections | 65853 | |
| <I/σ(I)> | 10.36 | 0.75 |
| Completeness [%] | 99.0 | 96 |
| Redundancy | 3.3 | 2.9 |
| CC(1/2) | 0.312 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM imidazole (pH 6.8), 200 mM lithium sulphate, and 4.5% polyethylene glycol 5000 MME |
