5MCB
Glycogen phosphorylase in complex with chlorogenic acid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-09-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.043 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.950, 128.950, 116.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.14021 |
| Rwork | 0.138 |
| R-free | 0.17744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jtu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 52193 | |
| <I/σ(I)> | 7.7 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 289 | 10mM BES Buffer |
