5MA5

GFP-binding DARPin fusion gc_K11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	58.710, 60.280, 90.240
Unit cell angles	86.94, 79.13, 89.35

Refinement procedure

Resolution	44.249 - 1.850
R-factor	0.1556
Rwork	0.154
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.249	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.044	0.654
Number of reflections	92746
<I/σ(I)>	18.31	2.46
Completeness [%]	89.5	89.2
Redundancy	3.74	3.76
CC(1/2)	0.999	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M tri Sodium citrate pH 5.6, 20% v/v 2-Propanol, 20%w/v PEG 4000