5M7R
Structure of human O-GlcNAc hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.230, 102.230, 285.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.250 - 2.350 |
| R-factor | 0.2166 |
| Rwork | 0.216 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cho |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.250 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.099 | 2.186 |
| Rmeas | 0.033 | |
| Rpim | 0.703 | |
| Number of reflections | 641548 | |
| <I/σ(I)> | 12 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 10 |
| CC(1/2) | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 0.14 - 0.2 M triammonium citrate pH 7.5, 16-20 % PEG 3350 |
