5M6X
Crystal Structure of human RhoGAP mutated in its arginine finger (R85A) in complex with RhoA.GDP.MgF3- human
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.470, 66.690, 76.890 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 76.550 - 2.400 |
| R-factor | 0.22666 |
| Rwork | 0.224 |
| R-free | 0.27197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w30 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.135 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.290 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.179 | 0.661 |
| Number of reflections | 23276 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 84.3 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 100 mM Bis Tris pH 5.5-6.5 20-25% (w/v) PEG3350 |
